Analysis of levels of G6P, lactate, and ATP in neutrophils of human G6PC3-deficient patients. Annexin V–depleted blood neutrophils were isolated from HD (n = 5; ages 23 to 37 years) and 2 G6PC3-deficient patients, P1 and P2, incubated for 30 minutes at 37°C in glucose-containing RPMI-1640 medium, and G6P, lactate, and ATP were determined as described in “G6P, lactate, and glycogen determination” and “Total ATP determination.” (A) Hema 3-stained cytospins of neutrophils. (B) G6P, lactate, and ATP levels. Data represent the mean ± SEM of 2 independent experiments, each done in duplicates. *P < .05; **P < .005. (C) Representative immunofluorescence of GLUT1 staining (green fluorescence), pan Cadherin membrane staining (red fluorescence), and DAPI nuclei staining (blue fluorescence) at 630× magnification.