American Society of Hematology

Figure 3

$Figure 3. CD41+ population is reduced in response to RapGEF2 deletion. (A-B) Bar graph showing the percentage of adherent RapGEF2+/+ and RapGEF2−/− E10.5 yolk sac (A) and embryonic cells (B) after 30 and 60 minutes of seeding in the fibronectin (FN)-coated plate. (C-D) Histogram displaying the in vitro migration levels of RapGEF2+/+ and RapGEF2−/− E10.5 yolk sac (C) and embryonic cells (D) after 6 and 12 hours. (E-I) Representative FACS images showing the distribution of Flk1+ (E), Flk1+CD41+ (F), c-Kit+CD34+ (G), c-Kit+CD45+ (H), and CD71+Ter119+ (I) fractions in the E10.5 RapGEF2+/+ and RapGEF2−/− yolk sacs. (J-N) Representative FACS images showing the distribution of Flk1+ (J), Flk1+CD41+ (K), c-Kit+CD34+ (L), c-Kit+CD45+ (M), and CD71+Ter119+ (N) in the E10.5 RapGEF2+/+ and RapGEF2−/− embryos.$

CD41⁺ population is reduced in response to RapGEF2 deletion. (A-B) Bar graph showing the percentage of adherent RapGEF2^+/+ and RapGEF2^−/− E10.5 yolk sac (A) and embryonic cells (B) after 30 and 60 minutes of seeding in the fibronectin (FN)-coated plate. (C-D) Histogram displaying the in vitro migration levels of RapGEF2^+/+ and RapGEF2^−/− E10.5 yolk sac (C) and embryonic cells (D) after 6 and 12 hours. (E-I) Representative FACS images showing the distribution of Flk1⁺ (E), Flk1⁺CD41⁺ (F), c-Kit⁺CD34⁺ (G), c-Kit⁺CD45⁺ (H), and CD71⁺Ter119⁺ (I) fractions in the E10.5 RapGEF2^+/+ and RapGEF2^−/− yolk sacs. (J-N) Representative FACS images showing the distribution of Flk1⁺ (J), Flk1⁺CD41⁺ (K), c-Kit⁺CD34⁺ (L), c-Kit⁺CD45⁺ (M), and CD71⁺Ter119⁺ (N) in the E10.5 RapGEF2^+/+ and RapGEF2^−/− embryos.

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