In vivo hematopoietic reconstitution is not altered by MLLDN. (A) Schematic representation of reconstitution experiment. The hematopoietic system of lethally irradiated mice was competitively reconstituted with unsorted lin⁻c-kit⁺ bone marrow cells transduced with either MigR1 or MigR1-MLLDN. Cd11b⁺ cells of the peripheral blood were analyzed for GFP positivity by flow cytometry every 4 weeks for 16 weeks. (B) GFP expression in the peripheral blood at 4 and 16 weeks postinjection of representative mice reconstituted with GFP⁺ bone marrow cells expressing either MigR1 or MigR1 MLLDN. The percentage of cells within the GFP⁺ gate is shown in blue. (C) The percentage of GFP⁺ cells from each individual mouse from the MigR1 (red) and MigR1 MLLDN (blue) cohort are plotted over time. BMT, bone marrow transplantation. (D) The percentage of GFP⁺ cells from whole bone marrow cells and LSK populations are plotted for each mouse from the MigR1 and MLLDN cohorts (mean shown by horizontal line; n = 7 (P > .05); unpaired 2-tailed Student t test). (E) Secondary transplantation assays performed by injecting primary transplanted bone marrow from the MigR1-702 and MLLDN-713 mice into 5 sublethally irradiated recipients for each group. GFP expression detected in the Cd11b⁺ peripheral blood cells is shown for each cohort through 16 weeks posttransplantation.