Vacuolin-1 increases enucleation in mouse fetal liver erythroblasts. (A) Effect of vacuolin-1 on enucleation of mouse fetal liver erythroblasts. The means ± standard deviations for 3 independent experiments are shown. *P < .05 and **P < .01. (B) Vacuolin-1 or its solvent were added to fetal liver enucleation assays at 38 hours and analyzed for effects on cell cycle, viability, and differentiation at 48 hours. Representative flow cytometric profiles are shown. (C) Model of erythroblast enucleation. An animated version is available as a supplemental video.