Granulysin recruits leukocytes in vivo. For in vivo recruitment experiments C57BL mice were injected intraperitoneally with PBS or 10 μg/mouse of 9-kDa GNLY (4 mice per group). Mice were killed, and the peritoneal cavity was rinsed with 5 mL of cold PBS, 5mM EDTA, 20 U/mL heparin. Cells in the peritoneal lavage were counted and stained to analyze the phenotype of the recruited cells by flow cytometric analysis after 4 hours of injection: (A) total number of cells, (B) CD11b/Gr1⁺/F480⁺, (C) CD11b/Gr1⁺/F480⁻, and at 24 hours (D) total number of cells, (E) CD11b/Gr1⁻/F480⁺, (F) CD11b/Gr1⁺/F480⁻, and (G) CD11b⁺/CD11c⁺. One representative experiment of 3 is shown. *P < .05, **P < .01.