Altered BM tissue of AA mouse model. AA mice were generated by infusion of B6-LN cells into sublethally irradiated C.B10 mice. BM whole-mount specimens of recipients were fluorescently stained using antibodies to CD8⁺ T cells (green), perlecan (white), and DAPI (nuclei) without or with BODIPY 493/503 (adipocytes), and subjected to examination on days 7, 10, and 17 by confocal microscopy (A,C). (A) After initial hypocellularity (day 7), a massive radial expansion of CD8⁺ T cells (green) was observed at day 10 and day 17 in parallel with extracellular matrix remodeling (white). Quantitative analysis of CD8⁺ cells in the BM samples was performed by flow cytometry (B). Concomitant staining of adipocytes (BODIPY in green), (C) revealed increasing number of adipocytes over time in parallel with extracellular matrix remodeling (white). (D) The cell numbers of WBCs, RBCs, and platelets in peripheral blood on days 7, 10, and 17 were enumerated by an automated cell counter. Scale bars are in micrometers. Related images appear in supplemental Video 6.