AREG secreted by BMDCs treated with ATPγS plus LPS promote LLC tumor growth. (A) Analysis of tumor volumes after coinjection of LLC cells and BMDC supernatants. BMDCs were treated with LPS or LPS+ATPγS during 24 hours, and their supernatants were incubated or not with an anti–mouse AREG blocking antibody for 3 hours. Tumor dimensions were measured at days 10, 12, and 14 after coinjection of LLC cells (5 × 105) and 100 μL of BMDC supernatants in C57BL/6 mice. Tumor volume was calculated using the standard formula: A × B2 × 0.52, where A is the longest diameter, and B is the shortest diameter. Results represent the mean ± SEM of 9 tumors at least for each condition. The Student t test was performed using Prism 5.0 software (**P < .01). (B) Effect of coinjection of LLC cells and BMDC supernatants on tumor weight. LLC tumor weight was measured at day 14 after coinjection of LLC (5 × 105) cells and BMDC supernatants. The Student t test was performed using Prism 5.0 software (***P < .001).
