Tumor-associated DCs express AREG and EGFR. (A) Tumor-associated CD11c+ cells express EGFR. Immunofluorescence analysis demonstrated that cells stained with CD11c (red) are also positive for EGFR (green) within tumor sections. Nuclei are stained with Hoechst (blue). Arrows point to CD11c and EGFR–double positive cells (40×/0.75 NA objective). (B) Tumor-associated CD11c+ cells express AREG. Immunofluorescence analysis demonstrated that cells stained with CD11c (red) are also positive for AREG (green) within tumor sections. Nuclei are stained with Hoechst (blue). Arrows point to CD11c and AREG–double positive cells (40×/0.75 NA objective).