Severe multilineage blocks in hematopoiesis in vav-cre NKAP cKO neonates. (A) Total thymocytes from wild-type (WT) and vav-cre NKAP cKO neonates were examined for surface expression of CD4 and CD8. Lineage-negative thymocytes from WT and vav-cre NKAP cKO neonates were examined for surface expression of c-Kit and CD25. (B) Total thymocyte number in wild-type (n = 13) or vav-cre NKAP cKO (n = 4). Results are shown on a logarithmic plot. A line indicated the average cellularity, and error bars reflect SD. (C) H&E staining of fixed sections of thymus from wild-type or vav-cre NKAP cKO neonates. (D) Neonatal liver was homogenized, and enriched for hematopoietic cells. Hematopoietic cells from WT and vav-cre NKAP cKO neonatal liver were analyzed for presence of B cells (CD19) and granulocytes (Gr-1/Mac1). (E) H&E sections of fixed sections of spleen from wild-type or vav-cre NKAP cKO neonates. (F) Total splenocytes from WT and vav-cre NKAP cKO neonates were analyzed for erythopoiesis by staining with CD71 and Ter119, and for megakaryocyte precursors by staining with CD41 and CD61. (G) CBCs with differential were analyzed on a Hemavet from 4 one-day-old vav-cre NKAP cKO and 20 wild-type littermates. In all of the parameters shown, the difference between WT and vav-cre NKAP cKO pups using the Student t test is significantly significant (P < .001). Data are shown as the average ± SEM.