NKAP-deficient HSCs exhibit decreased survival and proliferation. (A) At day 4 after a single injection of poly-IC, bone marrow LSK cells were analyzed for viability using DAPI and annexin V, as well as CD45.1 to separate SJL from WT littermate or Mx1-cre NKAP cKO hematopoietic progenitors. A representative FACS analysis is shown at right, and the average from 3 WT/SJL and 3 cKO/SJL mixed chimeras is shown at left. The Mx1-cre NKAP cKO LSKs are signicantly different from the other 3 samples, P < .01 by Student t test. Error bars represent SD. (B) At day 4 after a single injection of poly-IC, bone marrow LSK cells were analyzed for proliferation by BrdU incorporation, as well as CD45.1 to separate SJL from WT littermate or Mx1-cre NKAP cKO hematopoietic progenitors. BrdU was injected intraperitoneally 14 hours prior to killing. A representative FACS analysis is shown at right, and the average from 3 WT/SJL and 3 cKO/SJL mixed chimeras is shown at left. The Mx1-cre NKAP cKO LSKs are signicantly different from the other 3 samples, P < .04 by Student t test. Error bars represent SD from the mean.