Analysis of genes involved in proliferation and survival in NKAP-deficient HSCs. At day 4 after a single injection of poly-IC, bone marrow LSK cells from WT/SJL and (Mx1-cre NKAP) cKO/SJL mixed radiation chimeras were purified by FACS sorting. mRNA was generated, and examined by Q-PCR for expression of E2A, GATA2, Bmi1, cMyc, Hes1, cMyb, p19, p21 Cip1/Waf1, p27, Mcl1, and p53. Results shown are the average of triplicate amplifications from independent sorts from 2 mice in each group, normalized to 18S RNA. For each gene analyzed, expression was normalized to the expression in one of the replicates of WT littermate LSK. Other graphs (p53, phospho-p53 Ser15, Bcl-2, and Bcl-xL) show mean fluorescence intensity (MFI) from intracellular flow cytometry in LSK cells within each population, and the graphs are the average MFI in each population from 3 mice in each group. Error bars represent SD.