Validation of mechanosensitive genes by quantitative PCR. Total RNAs from intima of LCA or RCA at different time points (12, 24, and 48 hours) after ligation were collected. Differentially expressed genes were selected for quantitative PCR analyses based on 48-hour microarray results. Each RNA sample at each time was pooled from 3 different mouse carotids, representing a total of 9 (n = 3) to 15 (n = 5) mice. Microarray results for 12- and 48-hour time points are shown as fold increase (A) or fold decrease (C) of genes expressed in LCA over RCA in log2 scale (mean ± SEM; n = 3). All genes shown in the graphs were significant at FDR less than 10% at 48 hours. ‡Genes were also statistically significant (FDR < 10%) at 12 hours. Quantitative PCR validation results for 12-, 24-, and 48-hour time points are shown as fold increase (B) or fold decrease (D) of genes expressed in LCA over RCA in log2 scale (mean ± SEM; n = 3-5). All genes shown in the graphs were significant (P < .05) at 48 hours. *Genes were also statistically significant (P < .05) at 12 and 48 hours. (E) Six genes of interests that did not reach statistical significance (> 10% FDR) were examined by quantitative PCR. Data are mean ± SEM (n = 3). ‡< 10% FDR (LCA vs RCA). * < .05 (LCA vs RCA).