Disturbed flow in LCA decreases protein expression of Jam2 while up-regulating Angpt2, BMP4, and Lmo4. C57BL/6 mice underwent partial ligation, and LCA and RCA were collected 2 days after ligation. Paraffin sections were stained with specific antibodies for Jam2 (A), Angpt2 (B), BMP4 (C), and Lmo4 (D). Nuclei were counterstained with Hoechst (blue). Arrows indicate the protein expression in endothelial cells. L indicates lumen. Images are representative of n = 4 mice.