GPVI is cleaved in Adam10^−/− and Adam17^ex/ex mice in vivo. Control and mutant mice were injected intravenously with 100 μg of biotinylated anti-GPVI (JAQ1) antibody. Flow cytometric analysis of platelet count (A) and GPVI surface expression (B; indirectly: streptavidin-FITC) was performed. As a positive control in (B), wild-type platelets from untreated mice were incubated with 10 μg/mL biotinylated-JAQ1 in vitro and stained with streptavidin-FITC. (C) Mice were injected with 100 μg biotinylated-JAQ1, and plasma was collected at the indicated time points. Levels of GPVI-JAQ1^biotin complexes in plasma were determined by ELISA. Results of all experiments are mean ± SD (n = 4 mice per group, representative for 3 individual experiments).