Chromatin modification changes comparing CD34⁺ hematopoietic progenitors with AML blasts. (A) PCA identifies distinct patterns of H3K9me3 in AML versus CD34⁺ progenitors and WBCs. (B) Genomic regions differing in H3K9me3 patterns were hierarchically clustered and are indicated in this heatmap. Green represents higher H3K9me3 levels; and red, decreased H3K9me3 levels. (C) Number of genomic locations altered in H3K9me3 levels between AML and CD34⁺ specimens with regard to their distance from the TSS. A high number of core promoter regions (−400 to TSS) showed decreased H3K9me3 levels in AML.