34-1-2s induced a concentration-dependent pulmonary neutrophil accumulation. (A) BALB/c mice (●, N > 5) or SCID mice (○, N > 5) were injected with the indicated does of 34-1-2s mAb; and at 2 hours after injection, the mice were killed and their lungs were homogenized and the cells were loaded on to microslides by a cytospin and stained with hematoxylin and eosin. (B) BALB/c mice (●, N > 5) or SCID mice (○, N > 5) were injected with a 2.3-mg/kg dose of 34-1-2s mAb (determined from panel A; 50 μg); and at the indicated times, the mice were killed and their lungs were homogenized and the cells were loaded on to microslides by a cytospin and stained with hematoxylin and eosin. Data in both panels are percentage neutrophils counted within total nucleated cells.