Increased Mcl-1 expression in immune stimulated LTR-Tax CD4⁺ T cells. (A) Mcl-1 mRNA was quantitated in Tax and control CD4⁺ T cells 2 weeks after stimulation. mRNA levels were normalized to basal levels measured at time = 0. Values represent mean ± SEM of 3 separate experiments. (B) Mcl-1 protein expression by Tax and control CD4⁺ T cells at various times after activation was measured by Western blot analysis. Actin expression was measured as a housekeeping control.