Low (20×/0.40) and high (63×/0.75) magnification of liver histology before PIRR therapy and after the follow-up period from a responsive MC patient(female, 59 years). (A,C) Basal liver biopsy and (B,D) pictures from the second liver biopsy taken 3 years after the end of the treatment. Note a significant decline of inflammatory cells either in portal tract or in parenchyma. A sharp regression of hepatocyte fat degeneration was also noticed. In situ detection of CD20 protein with direct immunofluorescence was carried out in the first (E) and in the second (F) liver biopsy. A remarkable decline of CD20⁺ cells was demonstrated after PIRR therapy. Slides were viewed with a Leica DMBL research microscope using N-PLAN lens. Images were acquired using a Leica camera model DFC 490 and were processed with Leica application Version 2.4.OR1 (Leica Microsystems).