T2F8 transgene construct and genetic analysis. (A) Schematic diagram of transgene construct. T2F8 cassette (from 5′ to 3′): neomycin resistance, the murine Tie2 gene promoter (Tie2 promoter), chimeric intron, human B-domain deleted factor FVIII, poly A, and the Tie2 enhancer. (B) RT-PCR analysis shows that spliced T2F8 transgene mRNA (518 bp) is detected in the tissues from T2F8 transgenic mice. The housekeeping gene, HPRT, was used as an internal control (bottom panel).