Effect of Stat5b-CA TG expression on Teff phenotype and effector function. (A) CD4 and FoxP3 expression of freshly purified and pooled CD4⁺CD25⁻ T cells isolated from LNs of 10 WT (left) and 6 Stat5b-CA TG (right) mice. (B) Survival and weights of lethally irradiated B10.BR recipients injected with 10⁷ BM (*) and 2 × 10⁶ CD4⁺CD25⁻ T cells isolated from WT (▼) or Stat5b-CA TG mice (▽). P < .001, ▼ vs ▽. Pooled data are from 2 experiments; n = 16 mice/group total. (C) Flow cytometric analysis of freshly isolated WT and Stat5b-CA TG LN CD4⁺FoxP3⁻ T cells incubated with monoclonal antibody specific for the indicated antigen; representative data from 1 of 2 mice per group are shown.