Stat5b-CA TG expression supports Treg proliferation after transplantation into allogeneic hosts. (A) CD4 and FoxP3 expression of purified CD4+CD25− T cells isolated from lymph nodes of WT (left) and Stat5b-CA TG (right) mice. Pooled data are from the experiment shown in panel B; n = 10/group. (B) Lethally irradiated B10.BR recipients were injected with 107 BM cells and 5 × 106 CFSE-labeled CD4+CD25− T cells from WT or Stat5b-CA TG mice. (B-C) Splenocytes were isolated on day 5 after transplantation, and the percentage and absolute number of CD4+H-2Kb+FoxP3+ WT and Stat5b-CA TG T cells were determined. P values are as indicated. (D) The CFSE dilution profile was determined. (E) The percentage host (H-2Kb−CD4+FoxP3+) and percentage donor (H-2Kb+CD4+FoxP3+) T cells were analyzed. (B-E) Representative data from one mouse or pooled data from 2 independent experiments of 6 or 7 mice/group total are shown.
