Stat5b-CA TG expression impairs intrinsic Teff function but does not induce a Treg phenotype. (A) Survival and weights of lethally irradiated B10.BR recipients injected with 107 BM (*) and 1 × 106 sorted CD4+CD25−GFP− T cells from FoxP3-GFP-KI (WT; ▼) or FoxP3-GFP-KI × Stat5b-CA TG mice (TG; ▽) mice. P = .02, ▼ vs ▽. Pooled data are from 2 experiments; n = 16 mice/group total. (B) Histologic grade of GVHD in organs indicated on day 10 after transplantation in FoxP3-GFP-KI (WT; black) and FoxP3-GFP-KI × Stat5b-CA TG (Stat5b-CA TG; gray) recipients. **P < .01. *P < .05. Data are from 1 experiment with 4 and 7 or 8 mice/group. Splenocytes (C) and lamina propria cells (D) of recipients described in panel A were isolated on day 10, and the percentage FoxP3+(GFP+) cells was determined (panel C, 1 experiment with 4 and 5; and panel D, 4 and 8 mice per group, respectively). P values are as indicated. (E) The percentage TNF-α, IL-2, IFN-γ, IL-10, IL-17, or IL-4 expressing lamina propria CD4+ T cells isolated from the small intestine on day 10 after transplantation. P values are as indicated. Data are from 1 experiment with 4 and 7 or 8 mice/group.
