Phenotype characteristics of CD8⁺ after 6 days of coculture with either mDCs or MPA-DCs. Purified CD8⁺ T lymphocytes were cocultured for 6 days with allogeneic MPA-DCs or mDCs. At the end of coculture, cells were harvested and expression of CD25, CD69, CD28, CD152, CD154, CD103, CD134, and CD39 was assessed by FACS analysis for each condition: CD8⁺ mDCs (left column of each histogram) and CD8⁺ MPA-DCs (right column). Each point represents 1 experiment and the black bar represents the mean of 6 independent experiments. Wilcoxon test was used to calculate statistical differences between groups. The P value is indicated on each histogram of the figure. *P < .05.