Results of the ChIP assay. Native chromatin derived from BOECs was immunoprecipitated with antibodies against Ets-1, Ets-2, GATA-2, GATA-3 and GATA-6. Binding of the liver-specific transcription factor, HNF-1, known not to bind to this region was also evaluated. PCR was designed to amplify a 139-bp region (−111 to +28; a region containing the deletion mutation). The 2% agarose gel shows that amplified bands match the predicted PCR fragment size in relation to the DNA ladder. The VWF sequence in which the deletion is located binds clearly to Ets-2 and GATA-2. Weaker binding is seen with GATA-3 and GATA-6. Positive (RNA pol II) and negative (mouse IgG) controls produced the expected results (data not shown).