Figure 2
Figure 2. In vivo Ho uptake by BM myeloid progenitors. C57BL/6 mice were perfused with Ho dye intravenously 10 and 5 minutes before tissue sampling; BM cells were harvested on ice in the presence of verapamil and reserpine to block ATP-dependent transporters and stained for lineage, interleukin-7 receptor α (IL-7Rα), Sca-1, KIT, CD16/32, and CD34 surface antigens. (A) Representative dot plot of CD16/CD34 versus CD34 expression on viable 7-amino-actinomycin D− Lin− IL7-Rα− Sca-1− KIT+–gated BM cells and shows the gates representing common myeloid progenitors (CMPs), granulocyte/macrophage progenitors (GMPs), and megakaryocyte/erythrocyte progenitors (MEPs). (B-D) Representative dot plots of Ho blue fluorescence versus Ho red fluorescence of gated viable GMPs, MEPs, and CMPs, respectively. (E) Distribution of Hoechst bright, medium, and negative cells among CMPs, GMPs, and MEP. Data are average ± SD from 4 mice.

In vivo Ho uptake by BM myeloid progenitors. C57BL/6 mice were perfused with Ho dye intravenously 10 and 5 minutes before tissue sampling; BM cells were harvested on ice in the presence of verapamil and reserpine to block ATP-dependent transporters and stained for lineage, interleukin-7 receptor α (IL-7Rα), Sca-1, KIT, CD16/32, and CD34 surface antigens. (A) Representative dot plot of CD16/CD34 versus CD34 expression on viable 7-amino-actinomycin D− Lin− IL7-Rα− Sca-1− KIT+–gated BM cells and shows the gates representing common myeloid progenitors (CMPs), granulocyte/macrophage progenitors (GMPs), and megakaryocyte/erythrocyte progenitors (MEPs). (B-D) Representative dot plots of Ho blue fluorescence versus Ho red fluorescence of gated viable GMPs, MEPs, and CMPs, respectively. (E) Distribution of Hoechst bright, medium, and negative cells among CMPs, GMPs, and MEP. Data are average ± SD from 4 mice.

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