Relocation of HSCs and multipotent progenitors from nonperfused areas to highly perfused areas of the BM during G-CSF–induced mobilization of HSCs. 129SvJ mice were injected twice daily with saline (Sal) or G-CSF (G6) for 6 days. Ten and 5 minutes before tissue sampling, mice were perfused with Ho intravenously. After sampling, BM cells and endosteal cells were isolated and stained in the presence of verapamil and reserpine and analyzed for Ho dye uptake with the use of the previously defined gating strategies. Data show for each cell type the proportion of cells with maximal (Ho^bright, □), intermediate (Ho^med, ), and negative (Ho^neg, ■) Ho dye uptake. Data are mean ± SD of 3 mice for the saline group and 4 mice for the G-CSF group; ***P < .001 and *P < .05.