Immune reconstitution disease in Mycobacterium avium–infected T cell–deficient mice after CD4 T-cell adoptive transfer. (A) TCRα−/− mice were infected intravenously with 1 × 106 colony-forming units of M avium. After at least 2 months, 2 × 106 CD4 T cells isolated from uninfected WT mice were transferred intravenously. (B) Mice were monitored for weight loss (n > 11 mice/group) and survival (n = 8 mice/group). The shaded area between traveling error bars represents the SD. (C) Bacterial loads were measured in the lungs and spleens of control and T cell–recipient TCRα−/− mice on day 9 of transfer. (D) Lung function was assessed by measuring percent O2 saturation using a murine pulse oximeter on day 8 after transfer. Data are representative of at least 2 independent experiments performed, and the results shown in panel B have been repeated at least 15 times.
