Timecourse of uPA and PAI-1 mRNA and protein expression in 4T1 cells stimulated with FXa or thrombin. Levels of uPA mRNA (A) and protein (B) were determined by real-time PCR and ELISA, respectively. PAI-1 mRNA (C) and protein (D) induction were also measured using real-time PCR and ELISA. uPA and PAI-1 mRNA levels were normalized to HPRT mRNA. Results are shown as mean ± SEM of at least 3 independent experiments. *P ≤ .05 and **P ≤ .001 (control versus FXa treated). ^§P ≤ .05 and ^§§P ≤ .001 (control versus thrombin treated).