T-bet gene- and protein-expression levels after TLR2 stimulation. (A) Purified CD8 T cells were activated with plate-bound anti-CD3 antibody (1.5 μg/mL) in the presence or absence of TLR2 agonist (10 μg/mL) for the indicated time points. After these times, the mRNA levels of T-bet, IFN-γ, and granzyme B (+ SD) were determined by RT-PCR. (B) The protein levels of T-bet and EOMES in the nucleus were determined by Western blot analysis. The data shown in panel A are representative of 2 independent experiments, while the data shown in panel B are representative of 3 experiments, with each experiment showing identical trends.