American Society of Hematology

Figure 3

$Figure 3. TLR2 signals in CD8 T cells activate the mTOR pathway and are associated with increased T-bet biosynthesis. (A) CD8 T cells were activated with plate-bound anti-CD3 antibody (1.5 μg/mL) in the presence or absence of TLR2 ligand (10 μg/mL) for the time points indicated. The levels of phosphorylated or nonphosphorylated mTOR, 4E-EBP1, and P70S6K protein were determined by Western blot. (B) Forty-eight hours after activation, the levels of newly synthesized T-bet protein were determined in pulse-chase experiments. (C) Alternatively, the levels of newly synthesized T-bet RNA in TLR2-stimulated and nonstimulated T cells were determined in the different ribosomal fractions and quantified using RT-PCR. The data shown are representative of 3 independent experiments.$

TLR2 signals in CD8 T cells activate the mTOR pathway and are associated with increased T-bet biosynthesis. (A) CD8 T cells were activated with plate-bound anti-CD3 antibody (1.5 μg/mL) in the presence or absence of TLR2 ligand (10 μg/mL) for the time points indicated. The levels of phosphorylated or nonphosphorylated mTOR, 4E-EBP1, and P70S6K protein were determined by Western blot. (B) Forty-eight hours after activation, the levels of newly synthesized T-bet protein were determined in pulse-chase experiments. (C) Alternatively, the levels of newly synthesized T-bet RNA in TLR2-stimulated and nonstimulated T cells were determined in the different ribosomal fractions and quantified using RT-PCR. The data shown are representative of 3 independent experiments.

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