Minimally gained region on chromosome 16p (chr16: 1 480 000 - 26 000 000) is shown demarcated by the black bars. On the left side a representative profile with 16p gain is demonstrated (study case 19) showing overrepresentation of chromosomal material in the tumor DNA as a shift (at the BAC level) to the right from the neutral line. FISH validation of the finding is shown in the right panel with the use of probes derived from BAC clones: top left, study case 1 (interphase); bottom left, study case 19 (interphase); top right, KMH2 (interphase); and bottom right (metaphase). The red signal spans the ABCC1 locus on 16p; as references, a locus on 16q and a centromere 16 probe were used. FICTION: CD30 stain in light blue. Relative signal number gains of the ABCC1 locus are demonstrated.