GC-treated monocytes show increased resistance to apoptosis induced by different stimuli. Monocytes were cultured in the presence of 100nM DEX (GC) for 2 days or left untreated (Co). Spontaneous apoptosis was assessed after 24 hours (A-B) and serum withdrawal-induced apoptosis after 6 and 18 hours (C-D) by measurement of annexin V–positive cells (A and C, respectively) and cells containing hypodiploid nuclei (B,D, respectively).^15,16  Proportion of annexin V–positive cells was measured after 18 hours of anti-Fas–induced apoptosis. Nonspecific murine immunoglobulin M (IgM) served as control (E). Monocytes were cultured in the presence of different concentrations of DEX for 2 days. Subsequently, cells were challenged to apoptosis with CHX and ActD (F and G, respectively, ■) or left untreated (□). The amounts of annexin V–positive cells were measured after 18 hours of stimulation. Monocytes were cultured in the presence of 100nM DEX, MP, or T. After 2 days cells were stimulated with STS (■) or left untreated (□). The proportion of apoptotic cells was assessed by annexin V staining (H). Data show mean ± SEM (n = 3).