Tn-based targeting preferentially promotes B-cell and antibody responses. BALB/c mice (3 or 4 per group) were injected intradermally with 10 nmol of glycosylated multiple antigenic glycopeptide (MAG):Tn3-PV or unglycosylated multiple antigenic peptides PV peptide together with 25 μg of anti-CD40 antibody (A) or 10 μg of unmethylated cytosine-phosphate-guanosine (B). Control groups remained untreated or received αCD40 or unmethylated cytosine-phosphate-guanosine. Two to 3 weeks later, draining lymph node cells were analyzed for germinal center B cells by FACS (A and B). Serum samples were collected from immunized mice 2-3 weeks after intradermal immunization and anti-Tn antibody titers were determined (C-D). Data are expressed as the mean ± SD of 3 or 4 sera. Representative results from 2 experiments are shown.