Fli-1 gene deletion does not completely suppress the production of CD41-positive cells but decreases their maturation. (A-C) FACS diagrams showing CD41 expression in freshly purified Cre⁻ and Cre⁺ MEPs (A) and in their cell progenies taken after 5 days of culture in serum-free liquid medium (B) or after 7 days of culture in semisolid methocell medium (C) both supplemented with a complete cocktail of myeloid cytokines. (D) Histograms showing the progressive decrease in Cre⁺/Cre⁻ ratios of positive cells numbers according to increasing levels of CD41. Statistically significant differences between Cre⁺/Cre⁻ ratios are indicated by asterisks. All Cre⁺/Cre⁻ ratios were significantly different from 1 (n = 5 and n = 4 for liquid and methocell cultures, respectively).