Effects of R788 on leukemia cell distribution in blood, lymph nodes, and spleen. (A) B6/C3H mice with overt TCL1-002 leukemia were treated for 15 days with R788 or vehicle control. The number of circulating leukemia cells was determined on days 0, 4, 7, 10, and 15. On day 4, animals treated with R788 showed a greater increase in circulating leukemia cells than controls. Subsequently, the number of circulating leukemia cells increased dramatically in animals from the control group, whereas it remained stable in animals from the R788 group. The number of live animals available for analysis at each time point is indicated in the parentheses. (B) Four animals from each group were killed on day 5 to collect spleens and lymph nodes (inguinal and axillary) for analysis of tumor burden. Single-cell suspensions were prepared from the samples and counted on a Hemavet HV950FS hematology analyzer.