American Society of Hematology

Figure 2

$Figure 2. Quantitative RT-PCR validation of novel genes downstream of Spi1 identified in microarray data. (A) Relative expression levels in microarray. Values are mean ± SEM of all oligos for each gene present on the array. (B) Relative expression levels in quantitative RT-PCR. For normalization, peptidylprolyl isomerase A-like (ppial), which showed no changes over the mRNA samples used, was taken as reference. Results were analyzed using the ΔΔCt method. Gene expression values for spi1 MO (morpholino)-injected group were calculated relative to the phenol red-injected control group, whereas values for GFP+ cells were calculated relative to values of the GFP− cell fraction. Values of quantitative RT-PCR data are the mean ± SEM of 2 or 3 biologic replicates.$

Quantitative RT-PCR validation of novel genes downstream of Spi1 identified in microarray data. (A) Relative expression levels in microarray. Values are mean ± SEM of all oligos for each gene present on the array. (B) Relative expression levels in quantitative RT-PCR. For normalization, peptidylprolyl isomerase A-like (ppial), which showed no changes over the mRNA samples used, was taken as reference. Results were analyzed using the ΔΔC_t method. Gene expression values for spi1 MO (morpholino)-injected group were calculated relative to the phenol red-injected control group, whereas values for GFP⁺ cells were calculated relative to values of the GFP⁻ cell fraction. Values of quantitative RT-PCR data are the mean ± SEM of 2 or 3 biologic replicates.

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