Expression of genes mfap4, cxcr3.2, mpeg1, and ptpn6 does not colocalize with expression of neutrophil-specific marker mpx. Double fluorescent in situ with the mpx gene. Probes for mRNA are used as indicated in the bottom left corner of each image. Expression of genes, mfap4 (A-C), cxcr3.2 (D-F), mpeg1 (G-I), and ptpn6 (J-L) does not colocalize with expression of the mpx gene. Images show summed Z-stacks of consecutive confocal images through the posterior blood island of 28-hpf embryos (lateral view). In addition, no colocalization was observed in the head and yolk regions or in embryos at 48 hpf when mpx expression marks differentiated neutrophils (data not shown). Imaging conditions are as in Figure 4. Bars represent 20 μm.