Ad.DCs activate NK cells by cell-to-cell contact. DCs and NK cells were cultured in the same well or physically separated by a transwell membrane (TW). Ad.DCs were transduced using an MOI of 500. After 24 hours, (A) cell-surface expression of CD69 activation marker was measured by multicolor FACS analysis on CD56^dimCD16⁺ and CD56^highCD16⁻ NK cell subsets, and (B) cell culture supernatants were tested for secreted IFN-γ by multiplex cytokine assays. (A) Values are MFI for CD69 expressed on NK cells. (B) Values are mean ± SEM IFN-γ (pg/mL) of duplicate tests. The results shown in all panels are representative of 4 experiments.