Characteristics of protein-iPS cell derived from a different genetic strain from protein-donor ES cell. We further characterized FVB background sFB-protein-iPS cells. (A) RT-PCR for pluripotency-related genes. GAPDH was used as a loading control. (B) Scatterplots as determined by microarrays (n = 4, each). The global gene expression patterns were compared between the sFB and sFB-protein-iPS cell, and the ES cell and sFB-protein-iPS cell. The red lines indicate 2-fold changes in log scale. (C) ALP staining of sFB-protein-iPS cells. (D) Immunocytochemistry showing that sFB-protein-iPS cells express Nanog and Oct4. (E-F) In vitro differentiation potential of sFB-protein-iPS cells was evaluated by EB formation (E) and EB-based spontaneous differentiation (F). Immunocytochemistry revealed the expression of 3 germ layer marker proteins.