Selective expansions of distinct HIV-epitope–specific clonotypes into terminally differentiated effector-memory phenotypes (CCR7⁻/CD45RA⁺) and association of B27-KK10 tetramer binding with expression of distinct clonotypes and effector-memory differentiation. (A) Analysis of the TRBV populations representing single clonotypes of B8-FL8 tetramer⁺ cells by flow cytometry. (B) TRBV usage of distinct clonotypes and CCR7/CD45RO expression on B27-KK10 tetramer cells in subject 10022. (C) Pie charts comparing the relative T-cell memory distribution of B8-FL8⁺ and B27-KK10⁺ clonotypes identified by TRBV expression in subject 10022. (D) Frequency of clonotypes within the B27-KK10 tetramer⁺ population in subject 10022. (E) TRBV usage and CCR7/CD45RO expression on B27-KK10 tetramer dim and bright cells in subject 10022.