Efficiency of N-cad knockdown by shN-cad-1 and -2. (A) Western blotting revealed a reduction in N-cad protein abundance in NIH3T3 cells transfected with N-cad shRNAs but not with scramble shRNA. MNCD2, a rabbit anti–N-cad pAb (Abcam), and a mouse anti–N-cad mAb (clone 32, BD Biosciences) were used to detect N-cad protein. (B) Effects of shN-cad-1 and -2 on the expression of N-cad in LSK cells. After transduction of shN-cad-1 and -2, N-cad expression was analyzed. Quantitative real-time PCR analysis demonstrated that both shN-cad-1 and -2 significantly suppressed the expression of N-cad in LSK cells. Data are mean ± SD of 3 independent experiments. *P < .01. (C) Effects of shN-cad-1 and -2 on the expression of N-cad in LSK-CD34⁻ cells (LT-HSCs). The expression of N-cad in shRNA-transduced cells was analyzed by quantitative real-time PCR using a nanofluidic chip. Data are mean ± SD; n = 5. *P < .01.