N-cad knockdown enhances nuclear localization of β-catenin and expression levels of Lef-1. (A) Immunocytochemical staining of β-catenin (red). LSK cells were transduced with scramble shRNA, shN-cad-1, or shN-cad-2, and transplanted into lethally irradiated mice. One month after BMT, GFP⁺ LSK cells were sorted and stained with anti–β-catenin Ab. Scale bar represents 5 μm. (B) Percentage of GFP⁺ LSK cells showing nuclear β-catenin localization. Data are mean ± SD. *P < .01. (C) Immunocytochemical staining of Lef-1 (red). LSK cells were transduced with scramble shRNA, shN-cad-1, or shN-cad-2, and cultured on control-Fc– or N-cad-Fc–coated plates. After 3 days of culture, GFP⁺ cells were sorted and stained with anti-Lef1 Ab. The bottom panels represent higher power views of the boxed cells in the top panels. Scale bar represents 50 μm (top panels) and 5 μm (bottom panels). (D) Percentage of cells showing Lef1 expression in the nucleus. Data are mean ± SD. *P < .01.