N-cad function is required for the LTR activity of HSPCs. (A) Effects of N-cad shRNA on the LTR activity of HSPCs. The percentages of GFP⁺ donor-derived (Ly5.1⁺) cells in recipient mice 1 to 3 months after BMT are shown. Overexpression of shN-cad-1 and -2 significantly suppressed LTR of donor LSK cells. Data are mean ± SD (n = 10/group) from 3 independent experiments. (B) Representative FACS plots of PB analysis of shRNA-transduced donor cells 3 months after BMT.