FIP200 deletion led to increased mitochondrion mass and ROS in fetal hematopoietic cells. (A) Lysates from E14.5 liver of control or CKO mice were analyzed by Western blotting using anti-p62 (top) or anti-vinculin (bottom) antibodies. Asterisk on the right indicates the p62 band. (B-C) Relative mitochondrial mass (B) and ROS (C) of E14.5 fetal liver cells as measured by mean fluorescent intensity (MFI) of MitoTracker Green staining and DCF-DA staining, respectively. (D-E) Relative mitochondrial mass (D) and ROS (E) of myeloid cells from E14.5 fetal liver cells as measured by MFI of MitoTracker Green staining and DCF-DA staining, respectively. Representative FACS profiles of DCF-DA staining are shown on the right in panel E. (F-G) Relative mitochondrial mass (F) and ROS (G) of fetal HSCs from E14.5 embryos as measured by MFI of MitoTracker Green staining and DCF-DA staining, respectively. Representative FACS profiles of MitoTracker Green staining and DCF-DA staining are shown on the right.