Slow rolling of neutrophils on E-selectin and ICAM-1 requires Hck and Lyn as well as Fgr, lipid rafts, and Btk, but not PI3K. (A) Velocities of WT or SFK-deficient neutrophils rolling on E-selectin with or without coimmobilized ICAM-1. (B) Velocities of WT neutrophils rolling on E-selectin with or without coimmobilized ICAM-1 in the presence or absence of the vehicle control DMSO, MβCD or its inactive analog α-cyclodextrin (αCD), MβCD plus 15% FBS, or filipin III. (C) Velocities of WT neutrophils rolling on E-selectin with or without coimmobilized ICAM-1 in the presence or absence of the vehicle control DMSO, the PI3K inhibitor LY294002, or the Tec kinase inhibitor LFM-A13. (D) Velocities of WT, Btk^−/−, SHIP1^−/−, PI3Kγ^−/−, or PI3Kδ^−/− neutrophils rolling on E-selectin with or without coimmobilized ICAM-1. (E) Numbers of WT, SHIP1^−/−, PI3Kγ^−/−, or PI3Kδ^−/− neutrophils rolling or firmly adherent (arrest) on coimmobilized E-selectin, ICAM-1, and CXCL1 in the presence or absence of the PI3K inhibitor LY294002. In all experiments, the E-selectin density was 200 sites/μm² and the ICAM-1 density was 240 sites/μm². The wall shear stress was 1 dyne/cm². The data represent the mean ± SEM from 5 experiments. *P < .01.