E-selectin and P-selectin induce LFA-1–dependent slow rolling in human neutrophils. Whole human heparinized blood was perfused through flow chambers coated with E-selectin with or without ICAM-1 or P-selectin with or without ICAM-1. Rolling velocity on E-selectin (A) or P-selectin (B) with or without ICAM-1–coated flow chambers. PL-1 is a PSGL-1 blocking mAb. (C-D) Whole human blood was treated with anti–LFA-1 or anti-Mac-1 antibody (10 μg/mL for 20 minutes at room temperature[RT]) before perfusion through (C) E-selectin or E-selectin plus ICAM-1–coated flow chambers or (D) P-selectin or P-selectin plus ICAM-1–coated flow chambers. #P < .05 from column without antibodies. *P < .05 from selectin column. †P < .05 from selectin plus ICAM-1 column. Data presented are mean ± SEM and are representative of 3 independent experiments.