Induction of LFA-1 activation epitopes by fMLP. Whole blood neutrophils were stained with the pan-LFA-1 mAb TS1-22 (A), the LFA-1 extension reporter mAbs KIM127 (B), or NKI-L16 (C), or the high affinity reporter mAb 24 (D) with or without the fMLP stimulation for 5 minutes. Whole blood neutrophils were stained with anti–Mac-1 antibody (E) or anti–L-selectin antibody (F) before or after fMLP stimulation for 10 minutes. Data are representative of at least 10 independent experiments.