Time course of ATL146e effects in NY1DD mice. NY1DD mice were treated with osmotic minipumps infusing vehicle (■) or ATL146e (10 ng/kg/minute, osmotic pump, ▾). Pulmonary parameters were measured in NY1DD mice treated for 0, 1, 2, 3, or 7 days after the start of infusion. All parameters were maximally improved by 3 days after the start of ATL146e treatment, because no further changes were noted on day 7. The solid and dashed lines represent the mean ± SEM of parameters from congenic C57BL/6 mice. Data were analyzed by 1-way ANOVA with Neuman-Keuls posttesting. *P < .05 vs day 0.