ESRD patients' sera stimulate the production of functionally active TF in peripheral blood leukocytes from healthy donors. (A-B) The mPT values of supernatants of normal PMNs (A) and PBMCs (B) incubated with PBS (bar 1 in panels A and B), serum from healthy donors (HI, bar 2 in panels A and B), or ESRD sera isolated before (bar 3 in panels A and B; predialysis [Pred]) or during hemodialysis at various time points (bars 4-8 in panels A and B); mPT values of supernatants of PMNs (A) and PBMCs (B) either incubated with APS serum (bar 10 in panels A and B) or incubated with ESRD sera collected between 15 and 30 minutes after the beginning of hemodialysis and treated with TF monoclonal antibodies (bar 11 in panels A and B). Data are representative of 10 independent experiments (mean mPT values ± SD). A paired t test was applied to examine statistical significance: *P < .05; **P < .001. (C) The relative expression of TF mRNA in normal PMNs cultured in the presence of PBS or ESRD patients' sera isolated before or during hemodialysis. Data are representative of 6 independent experiments (mean fold expression [2^−DDCt analysis] ± DC_t SD). DDCt indicates Delta Delta Cycle threshold; and DCt, Delta Cycle threshold. The Wilcoxon matched-pairs test was used to assess statistical significance; *P < .01. Horizontal lines above data bars indicate statistical significance.