Procoagulant properties of ESRD sera are complement-dependent. (A) The amounts of soluble TCC in plasma from healthy donors (HI, bar 1) or ESRD patients before dialysis (predialysis [Pred], bar 2), and during (bars 3-6) hemodialysis. Data are representative of one experiment (mean ± SD of 4 persons per group). The Mann-Whitney test and paired t test were applied to assess statistical significance: *P < .05. (B-C) The expression of C5a receptor on the surface of PMNs (B) or in permeabilized cells (C) from healthy donors (HI), ESRD patients before dialysis (predialysis [Pred]), and during hemodialysis (5, 30, 60, and 120 minutes). The results are presented as mean fluorescent intensity (MFI) ratios of the cells stained with C5aR monoclonal antibodies to the cells stained with the isotype control. Data are representative of one experiment (mean ± SD of 4 healthy donors and 3 ESRD patients). The unpaired and paired t tests were used to assess statistical significance: *P < .05. (D) The mPT values of supernatants of normal PMNs treated with sera from healthy donors (HI, bar 1), ESRD patients before (predialysis [Pred], bar 2), and during (bar 3) hemodialysis or preincubated with C5a (bar 4) or C3a (bar 5) receptor antagonists and then treated with sera obtained from ESRD patients during hemodialysis. Data are representative of 6 independent experiments (mean ± SD). The Wilcoxon matched-pairs test was used to assess statistical significance: *P < .001. (E) The relative expression of TF mRNA in normal PMNs treated with sera obtained from ESRD patients during hemodialysis or preincubated with C5aR antagonist and then treated with sera from the same patients. Data are representative of 2 independent experiments (mean ± SD of 3 patients). The Wilcoxon matched-pairs test was used to assess statistical significance: P < .01. Horizontal lines above data bars indicate statistical significance.